Cycling can be used to solve the increasingly serious traffic problem in most cities. On-road bike lane is useful to improve the safety of cyclists and the travel share of bike. This will, however, reduce the space of motor vehicles. This paper puts forward one bi-level programming model to describe how to share the motorway between cyclists and motorists. In the upper level, the trade-off is discussed in point of transport authority, with the constraint of budget and connectivity. Then, bike flow and vehicle flow are assigned by user equilibrium in the lower level. One genetic algorithm is designed to solve this model, which is verified by one numerical example.